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Size:50ul  100ulConcentration:1mg/mlApplications:WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000Cross Reactive Species:Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Dog)For research use only. Not intended for diagnostic or therapeutic use.Host:RabbitTarget Protein:SEMA4AIR:Immunogen Range:361-460/761Clonality:PolyclonalIsotype:IgGEntrez Gene:64218Swiss Prot:Q9H3S1Source:KLH conjugated synthetic peptide derived from human SEMA4A:361-460/761 Purification:affinity purified by Protein AStorage:0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.Background:SEMA4A belongs to the semaphorin family of soluble and transmembrane proteins. These proteins play a role in guidance of axonal migration during neuronal development and in

人腦源性神經(jīng)營(yíng)養(yǎng)因子(BDNF)ELISA檢測(cè)試劑盒  ELISA試劑盒技術(shù)流程  雙抗體夾心法(檢測(cè)未知抗原)間接法(檢測(cè)未知抗體)1、用緩沖液將抗體稀釋至1-10ug/ml。在反應(yīng)孔中加0.1ml，4℃ 過(guò)夜。次日，棄去孔內(nèi)溶液，用洗滌緩沖液洗3次。2、加樣：加一定稀釋的待檢樣品0.05ml于上述已包被之反應(yīng)孔中，置37℃ 孵育1小時(shí)。然后洗滌(同時(shí)做空白孔，陰性對(duì)照孔及陽(yáng)性對(duì)照孔)。3、加酶標(biāo)抗體：于各反應(yīng)孔中，加入新鮮稀釋的酶標(biāo)抗體(經(jīng)滴定后的稀釋度)0.05ml。37℃ 孵育0.5～1小時(shí)，洗滌。4、加底物液顯色：于各反應(yīng)孔中加入臨時(shí)配制的TMB底物溶液0.1ml，37℃ 10～30分鐘。5、終止反應(yīng)：于各反應(yīng)孔中加入2M硫酸0.05ml6、結(jié)果判定：可于白色背景上，直接用肉眼觀(guān)察結(jié)果：反應(yīng)孔內(nèi)顏色越深，陽(yáng)性程度越強(qiáng)，陰性反應(yīng)為無(wú)色或極淺，依據(jù)所呈顏色的深淺，以“+”、“-”號(hào)表示。也可測(cè)OD值：在ELISA檢測(cè)儀上，于450nm(若以ABTS顯色，則410nm)處，以空白對(duì)照孔調(diào)零后測(cè)各孔OD值，若大于規(guī)定的陰性對(duì)照OD值的2.1倍，即為陽(yáng)性。1、用包被緩沖液將已知抗原稀釋至1-10ug/ml， 每孔加0.1ml，4℃過(guò)夜。次日洗滌3次。2、加樣：加一定稀釋的待檢樣品(未知抗體)0.05ml于上述已包被之反應(yīng)孔中,置37℃孵育1小時(shí),洗滌。(同時(shí)做空白、陰性及陽(yáng)性孔對(duì)照)3、加酶標(biāo)抗體：于反應(yīng)孔中，加入新鮮稀釋的酶標(biāo)第二抗體(抗抗體)0.05ml，37℃孵育30-60分鐘，洗滌,＊后一遍用DDW洗滌。4、加底物液顯色：于各反應(yīng)孔中加入臨時(shí)配制的TMB底物溶液0.1ml，37℃ 10～30分鐘。5、終止反應(yīng)：于各反應(yīng)孔中加入2M硫酸0.05ml6、結(jié)果判定：可于白色背景上，直接用肉眼觀(guān)察結(jié)果：反應(yīng)孔內(nèi)顏色越深，陽(yáng)性程度越強(qiáng)，陰性反應(yīng)為無(wú)色或極淺，依據(jù)所呈顏色的深淺，以“+”、“-”號(hào)表示。也可測(cè)OD值：在ELISA檢測(cè)儀上，于450nm(若以ABTS顯色，則410nm)處，以空白對(duì)照孔調(diào)零后測(cè)各孔OD值，若大于規(guī)定的陰性對(duì)照OD值的2.1倍，即為陽(yáng)性。 ELISA試劑盒需自備的設(shè)備及試劑    1. 450±10nm濾光片的酶標(biāo)儀(建議儀器使用前提前預(yù)熱)2. 單道或多道微量加液器及吸頭3. 稀釋樣品的EP管4. 蒸餾水或去離子水5. 吸水紙6. 盛放洗液的容器 性能:1．準(zhǔn)確性：標(biāo)準(zhǔn)品線(xiàn)性回歸與預(yù)期濃度相關(guān)系數(shù)R值，大于等于0.9900。2．靈敏度：檢測(cè)濃度小于10 pg/ml。3．特異性：不與其它可溶性結(jié)構(gòu)類(lèi)似物交叉反應(yīng)。4．重復(fù)性：板內(nèi)、板間變異系數(shù)均小于15%。ELISA試劑盒優(yōu)點(diǎn)：1、高效、靈敏、特異的抗體；2、重復(fù)性和可靠性高；3、吸附性能好，空白值低，孔底透明度高的固相載體；4、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類(lèi)型；5、可檢測(cè)指標(biāo)齊全：細(xì)胞因子檢測(cè)、心肌梗塞檢測(cè)、內(nèi)分泌檢測(cè)、肝纖維化檢測(cè)、自身免疫檢測(cè)、腫瘤標(biāo)志物檢測(cè)、傳染病檢測(cè)、特種蛋白檢測(cè)、優(yōu)生優(yōu)育檢測(cè)等等；6、價(jià)格優(yōu)惠，質(zhì)量保證，限度的節(jié)省實(shí)驗(yàn)經(jīng)費(fèi)。 實(shí)驗(yàn)步驟1. 使用前將所有的試劑和標(biāo)本緩慢均衡至室溫(18-25oC)，試劑不能直接在37oC溶解。2. 標(biāo)準(zhǔn)品(凍干品)：每瓶標(biāo)準(zhǔn)品加入標(biāo)準(zhǔn)品稀釋液1mL，蓋好后室溫靜置大約10分鐘，同時(shí)反復(fù)顛倒/搓動(dòng)以助溶解，其濃度為2000pg/mL。準(zhǔn)備7個(gè)稀釋標(biāo)準(zhǔn)品的EP管，每個(gè)EP管中加入150μL的標(biāo)準(zhǔn)品稀釋液，如圖所示依次倍比稀釋成不同的梯度， 標(biāo)準(zhǔn)品稀釋液(0pg/mL)直接作為空白孔。為保證實(shí)驗(yàn)結(jié)果有效性，每次實(shí)驗(yàn)請(qǐng)使用新的標(biāo)準(zhǔn)品溶液。3. 濃洗滌液：用580mL蒸餾水或去離子水將20mL濃洗滌液稀釋至600mL，進(jìn)行30倍稀釋。 注意事項(xiàng)劑盒的儲(chǔ)存及有效期1. 未開(kāi)封的試劑盒：所有試劑均按試劑瓶標(biāo)簽上所示保存。請(qǐng)注意，收到試劑盒后請(qǐng)盡快將TMB 洗滌液，終止液保存于4℃，其他試劑保存于-20℃。2. 使用后的試劑盒：剩余試劑仍需按照試劑瓶標(biāo)簽所示的溫度保存，開(kāi)封后的酶標(biāo)板要加干燥劑后密封保存于-20℃，避免潮濕。 須知：試劑盒內(nèi)酶標(biāo)條可拆卸，按實(shí)驗(yàn)需求可分多次使用；使用后的剩余試劑盒建議在首次實(shí)驗(yàn)后1個(gè)月內(nèi)使用完畢。產(chǎn)品過(guò)期時(shí)間以盒子上的標(biāo)簽為準(zhǔn)，保質(zhì)期內(nèi)所有組分都確保是穩(wěn)定的。 ELISA試劑盒報(bào)1、ELISA標(biāo)準(zhǔn)曲線(xiàn)；2、詳細(xì)的實(shí)驗(yàn)步驟；3、完整的實(shí)驗(yàn)報(bào)告（試劑耗材，實(shí)驗(yàn)方法，實(shí)驗(yàn)結(jié)果及結(jié)果說(shuō)明）； [樣本處理及要求]1.  血清：將收集于血清分離管的全血標(biāo)本在室溫放置2小時(shí)或4℃過(guò)夜，然后1000×g離心20 分鐘，取上清即可，或?qū)⑸锨逯糜?20℃或-80℃保存，但應(yīng)避免反復(fù)凍融。2.  血漿：用EDTA或肝素作為抗凝劑采集標(biāo)本，并將標(biāo)本在采集后的30分鐘內(nèi)于2-8℃ 1000×g離心15分鐘，取上清即可檢測(cè)，或?qū)⑸锨逯糜?20℃或-80℃保存，但應(yīng)避免反復(fù)凍融。3.  組織勻漿：用預(yù)冷的PBS (0.01M, pH=7.4)沖洗組織，去除殘留血液（勻漿中裂解的紅細(xì)胞會(huì)影響測(cè)量結(jié)果），稱(chēng)重后將組織剪碎。將剪碎的組織與對(duì)應(yīng)體積的PBS（一般按1:9的重量體積比，比如1g的組織樣品對(duì)應(yīng)9mL的PBS，具體體積可根據(jù)實(shí)驗(yàn)需要適當(dāng)調(diào)整，并做好記錄。推薦在PBS中加入蛋白酶抑制劑）加入玻璃勻漿器中，于冰上充分研磨。為了進(jìn)一步裂解組織細(xì)胞，可以對(duì)勻漿液進(jìn)行超聲破碎，或反復(fù)凍融。＊后將勻漿液于5000×g離心5~10分鐘，取上清檢測(cè)。4.  細(xì)胞培養(yǎng)物上清或其它生物標(biāo)本：請(qǐng)1000×g離心20分鐘，取上清即可檢測(cè)，或?qū)⑸锨逯糜?20℃或-80℃保存，但應(yīng)避免反復(fù)凍融。注：標(biāo)本溶血會(huì)影響＊后檢測(cè)結(jié)果，因此溶血標(biāo)本不宜進(jìn)行此項(xiàng)檢測(cè)。 [需要而未提供的試劑和器材]1.酶標(biāo)儀（450nm）2.高精度加樣器及槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3.37℃恒溫箱4.蒸餾水或去離子水 ELISA檢測(cè)試劑盒承諾公司長(zhǎng)期與各大高校、醫(yī)院及權(quán)威科研單位保持著良好的合作關(guān)系，公司的產(chǎn)品質(zhì)量及服務(wù)態(tài)度得到了他們的一致認(rèn)可。篤瑪生物本著客戶(hù)至上的原則為客戶(hù)提供優(yōu)質(zhì)產(chǎn)品，公司承諾產(chǎn)品有任何質(zhì)量的問(wèn)題免費(fèi)包退換（非人為因素造成的）。公司提供免費(fèi)代測(cè)服務(wù)，并且承諾收到樣本七個(gè)工作日出結(jié)果，確保數(shù)據(jù)的準(zhǔn)確性

Overview Cat. No： DM-11A04-G Format： LiquidHost： Homo Class： MonoclonalType： Antibody Specificity： Binding to RSV F protein Concentration： Please check the label on the tube. Purity ≥95% as determined by SDS-PAGE Purification： Protein A Storage buffer： PBS or Tris-Gly Storage conditions： Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Warning ? Not for therapeutic use.

Overview Cat. No:DM-A11A04-M Format Liquid Host Homo Class Monoclonal Type Antibody Specificity Binding to RSV F protein Concentration Please check the label on the tube. Purity ≥95% as determined by SDS-PAGE Purification / Storage buffer PBS or Tris-Gly Storage conditions Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Warning ? Not for therapeutic use.

OverviewCat. No:DM-11A04-G Format: Liquid Host: HomoClass: MonoclonalType: AntibodySpecificity: Binding to RSV F proteinConcentration: Please check the label on the tube. Purity: ≥95% as determined by SDS-PAGE Purification Protein A Storage buffer: PBS or Tris-Gly Storage conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.Warning ? Not for therapeutic use.

快速高爾基染色試劑盒(神經(jīng)元和膠質(zhì)細(xì)胞)產(chǎn)品貨號(hào):  DM1158 產(chǎn)品規(guī)格:  6 × 125ml/6 ×250ml產(chǎn)品簡(jiǎn)介:Golgi-Cox浸染法是研究神經(jīng)元和膠質(zhì)細(xì)胞正常和非正常形態(tài)＊有效的方法之一。 使用Golgi技術(shù)，在藥物處 理過(guò)的動(dòng)物腦中和因神經(jīng)疾病死亡的病人腦中發(fā)現(xiàn)了神經(jīng)樹(shù)突和樹(shù)突微小的形態(tài)改變 。然而Golgi染色法的不可 靠性且費(fèi)時(shí)已成為這種方法廣泛應(yīng)用的障礙?？焖俑郀柣旧噭┖校ㄉ窠?jīng)元和膠質(zhì)細(xì)胞） 是根據(jù)Ramón-Moliner ，Glaser和Van der Loos所闡述的方法原 理設(shè)計(jì)的 。該試劑盒不僅極大地改進(jìn)并簡(jiǎn)化了Golgi-Cox技術(shù)，而且被證實(shí)在顯示神經(jīng)元和膠質(zhì)細(xì)胞，尤其是樹(shù) 突棘的形態(tài)細(xì)節(jié)極為靈敏可靠。 生物生產(chǎn)的快速高爾基染色試劑盒已被廣泛用于數(shù)種動(dòng)物腦組織染色。 產(chǎn)品組成: 產(chǎn)品名稱(chēng)6 × 125ml6×250ml溶液A125ml250ml溶液B125ml250ml溶液C125ml×2250ml×2溶液D125ml250ml溶液E125ml250ml注：溶液C啟封后請(qǐng)盡快用完。 需要但未包括在試劑盒里的物品1.      雙蒸水或Milli-Q水2.      塑料/玻璃管或瓶 、琉璃樣品回收器、天然毛畫(huà)筆、滴瓶、塑料鑷子3.      組織學(xué)耗材：明膠包被的顯微鏡載玻片蓋玻片、染色罐乙醇二甲苯樹(shù)膠封片劑光學(xué)顯微鏡 組織制備使用此試劑盒前必須仔細(xì)閱讀以下說(shuō)明??！l 所用容器（＊好為塑料材質(zhì)） 必須用蒸餾水沖洗干凈。l 當(dāng)溶液A和溶液B存在時(shí)不能使用金屬器具。l 保持容器密封。l 用溶液A和溶液B處理的組織和切片，應(yīng)該避光。l 除非特別指出， 以下流程需在室溫下完成。  快速高爾基染色試劑盒(神經(jīng)元和膠質(zhì)細(xì)胞) 1.      殺死動(dòng)物之前對(duì)實(shí)驗(yàn)動(dòng)物進(jìn)行深度麻醉。應(yīng)盡快地從顱骨中取出動(dòng)物的腦（或死去病人的腦），但操作時(shí)必 須非常小心以免損傷或壓迫組織。注意l 除非完全必要，不要對(duì)動(dòng)物進(jìn)行灌注。如果確實(shí)需要對(duì)動(dòng)物進(jìn)行灌注（用4%的多聚甲醛處理不要超過(guò)5分鐘）， 一定不要對(duì)組織進(jìn)行后固定處理。l 體積較大的腦部樣本包括大鼠腦部應(yīng)該用鋒利的刀片切成大約10mm厚的塊狀。重要提示！2.      用雙蒸水或Milli-Q水快速?zèng)_掉組織表面的血液。3.      把組織浸泡在由溶液A和B等體積混合的浸漬液中，室溫下黑暗保存兩周* 。浸泡6個(gè)小時(shí)以后或次日更換新 的浸漬液。*對(duì)于大多數(shù)情況浸泡2周是足夠的。然而，不同的組織類(lèi)型及組織的大小不同可能需要延長(zhǎng)或縮短浸泡時(shí)間來(lái)達(dá) 到＊佳效果。對(duì)于每種類(lèi)型的組織的浸泡時(shí)間應(yīng)該通過(guò)試驗(yàn)獲得，但是對(duì)于大多數(shù)組織浸泡3周是足夠的。注意， 延長(zhǎng)浸泡時(shí)間可能增加染色背景。注意l 溶液A和溶液B的混合液至少在用之前24小時(shí)配制，并不能攪拌。l 采用以上方案則不會(huì)產(chǎn)生沉淀是關(guān)鍵的。l 制備好的浸泡液在用之前室溫黑暗保存可長(zhǎng)達(dá)一個(gè)月。l 每cm3 待研究組織至少用5ml浸泡液。注意用較少的浸泡液可能降低染色的靈敏性和可靠性。l 為取得＊佳結(jié)果，在浸泡期間每周兩次對(duì)裝有組織的容器可輕輕地左右旋渦（一定不要晃動(dòng)！ ）幾秒鐘。Warning溶液A和B（含有升汞，重鉻酸鉀和鉻酸鉀） 接觸皮膚是有毒的，如果吞咽可能是致命的。實(shí)驗(yàn)應(yīng)該在化學(xué)通風(fēng) 櫥中完成。當(dāng)操作這些試劑時(shí)應(yīng)穿戴防護(hù)服，手套和防護(hù)面具/眼鏡。不要把溶液A和B的廢棄物倒進(jìn)水槽中！把溶液A和B的廢棄物收集起來(lái)放到一個(gè)瓶子中，致電安全辦公室或有執(zhí)照的專(zhuān)業(yè)廢物處理服務(wù)的部門(mén)處理些材 料。4.      轉(zhuǎn)移組織到溶液C中室溫黑暗至少72小時(shí)（可長(zhǎng)達(dá)1周） 。24小時(shí)后或次日至少更換一次溶液。5.      在-20℃到-22℃的條件下用冷凍切片機(jī)將組織切成100-200um厚的薄片，也可用其它型號(hào)的顯微鏡薄片切片 機(jī)包括滑動(dòng)切片機(jī)和振動(dòng)切片機(jī) 。用樣本回收器把樣本轉(zhuǎn)移到含有溶液C的明膠包被的顯微鏡載玻片上。載 玻片上多余的溶液用吸管吸走并用濾紙吸干（載玻片上的溶液必須盡可能地吸干否則切片將從載玻片上脫落 下來(lái)） 。切片可以室溫下自然風(fēng)干（不能用電風(fēng)扇或電熱板使其干燥） 。為取得＊佳結(jié)果，應(yīng)盡快地完成切 片，制備好的切片如果保存于載玻片盒中，室溫黑暗條件下＊長(zhǎng)可保存3天。注意l 為避免組織受冷凍切片機(jī)的損害，并盡可能地保持細(xì)胞形態(tài)學(xué)，組織在進(jìn)行冷凍切片之前應(yīng)快速冰凍。 比如， 組織應(yīng)按以下描述盡可能地快速冰凍：把組織放在一個(gè)塑料匙中慢慢地浸入含有干冰的異戊烷中預(yù)冷（為取 得＊佳結(jié)果，溫度應(yīng)保持在-70℃以下并且浸入過(guò)程用時(shí)1分鐘，越慢越好） 。組織完全浸入異戊烷之后，使 組織在異戊烷中浸幾秒鐘，然后再放置干冰上1分鐘以確保組織能很好地冰凍。進(jìn)行切片之前不要讓組織融 化。l 切片機(jī)的型號(hào)可能會(huì)有不同，但所用的型號(hào)確保能切厚的切片（比如100um）。l 如果冰凍切片機(jī)只有一個(gè)溫度設(shè)置，那么在進(jìn)行切片之前把溫度設(shè)置在-22℃至少4個(gè)小時(shí)。如果有兩個(gè)溫度 設(shè)置，那么設(shè)置槽內(nèi)溫度比樣本溫度低1℃ 。請(qǐng)注意大多數(shù)情況下-22℃是＊佳的溫度，然而，為了更順利地 切出切片并沒(méi)有破碎，不同型號(hào)的切片機(jī)和不同的組織可能需要更高或更低的槽內(nèi)溫度。l 對(duì)于用冰凍切片機(jī)切片， 以下幾點(diǎn)提示是有益的： 1 ）用蒸餾水把組織固定于樣本盤(pán)上，但必須確保組織沒(méi) 有融化（可以在干冰上完成） 。組織可以被固定在任何類(lèi)型的組織冰凍介質(zhì)上包括OCT ，但要避免切斷介質(zhì)。 不要在OCT中包埋組織，如果組織確實(shí)需要包埋，用TFM替代OCT 。2） 組織固定到樣本盤(pán)后，放置組織于   快速高爾基染色試劑盒(神經(jīng)元和膠質(zhì)細(xì)胞) 干冰上10分鐘，然后立即把固定有樣本的樣本盤(pán)安裝到冰凍切片機(jī)上，等5分鐘之后進(jìn)行切片。3）切好的一 些切片（不要使用防卷板） ，如果組織溫度過(guò)低比如切片顯示出與刀片平行的裂縫，先等幾分鐘再進(jìn)行下一 個(gè)切片，否則可繼續(xù)切。l 浸泡的腦組織可用瓊脂糖或明膠包埋然后用冰凍切片機(jī)切片。然后收集切片時(shí)（充滿(mǎn)切片槽） ，必須使用溶 液C 。否則切片易干燥裂紋。l 如果用滑動(dòng)切片機(jī)切浸泡的組織，樣本盤(pán)和刀片都需維持在較低的溫度（0℃以下） 按照操作手冊(cè)的描述切 片固定在含有溶液C的載玻片上是重要的。VI.  染色步驟在染色過(guò)程中和蓋蓋玻片之前的任何一步都不要讓切片變干1.      用雙蒸水或Milli-Q水沖洗切片兩次，每次4分鐘。2.      把切片置于由1份溶液D ， 1份溶液E和2份的雙蒸水或Milli-Q水組成的混合液中10分鐘。 比如：溶液D          10ml                                    溶液E        10ml                          雙蒸水       20ml注意l 工作液＊好現(xiàn)配現(xiàn)用，每100ml工作液＊多用于100張切片（比如小鼠腦） ， 根據(jù)切片的大小。l 盛有工作液的瓶子或染色罐必須蓋好蓋子防止試劑蒸發(fā)。l 為取得＊佳結(jié)果，孵育期間應(yīng)頻繁攪拌工作液。3.      用蒸餾水或Milli-Q水沖洗切片兩次，每次4分鐘（蒸餾水應(yīng)頻繁更換新的）。4.      用結(jié)晶紫或硫堇對(duì)切片進(jìn)行復(fù)染（可選步驟）。注意l 對(duì)于復(fù)染，延長(zhǎng)步驟3的時(shí)間， 比如用沖洗4次每次5分鐘代替原來(lái)的沖洗2次每次4分鐘，或者更長(zhǎng)的時(shí)間。5.     在50% ，75%和95%的乙醇中對(duì)切片進(jìn)行脫水，每個(gè)濃度梯度脫水4分鐘（不要跳過(guò)任何一個(gè)步驟）。6.      然后在無(wú)水乙醇中對(duì)切片進(jìn)行脫水，4次，每次4分鐘（不要延長(zhǎng)時(shí)間）。7.      在二甲苯中透明，3次，每次4分鐘，并且用樹(shù)脂封片劑對(duì)蓋玻片進(jìn)行封片。注意l 蓋玻片之前切片可以暫時(shí)存于二甲苯中幾個(gè)小時(shí)。l 為取得＊佳結(jié)果，＊好用未稀釋的封片劑。l 用Golgi染色的切片無(wú)論何時(shí)都應(yīng)避光保存。 注意事項(xiàng)1.      快速高爾基染色試劑盒（神經(jīng)元和膠質(zhì)細(xì)胞）僅用于體外研究，不能用于診斷或其它應(yīng)用。2.     試劑盒所包含有試劑是有毒的，吸入或接觸皮膚是有害的，如果吞咽可能是致命的。不要用嘴吸。避免吸入 和接觸皮膚和眼睛。如果接觸，立即用大量的水沖洗并求助醫(yī)生。3.      在化學(xué)通風(fēng)櫥下完成實(shí)驗(yàn)。操作這些試劑時(shí)須穿戴合適的防護(hù)服，手套和眼睛和面部防護(hù)罩，完成實(shí)驗(yàn)之后 把手徹底沖洗干凈。 保存條件:  室溫，有效期 12 個(gè)月。         上海篤瑪生物科技有限公司

本試劑盒只能用于科學(xué)研究，不得用于醫(yī)學(xué)診斷人（Human）白細(xì)胞介素2（IL-2）ELISA檢測(cè)試劑盒使用說(shuō)明書(shū)檢測(cè)原理試劑盒采用雙抗體一步夾心法酶聯(lián)免疫吸附試驗(yàn)（ELISA）。往預(yù)先包被白細(xì)胞介素2（IL-2）抗體的包被微孔中，依次加入標(biāo)本、標(biāo)準(zhǔn)品、HRP標(biāo)記的檢測(cè)抗體，經(jīng)過(guò)溫育并徹底洗滌。用底物TMB顯色，TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成＊終的黃色。顏色的深淺和樣品中的白細(xì)胞介素2（IL-2）呈正相關(guān)。用酶標(biāo)儀在450nm 波長(zhǎng)下測(cè)定吸光度（OD 值），計(jì)算樣品濃度。樣品收集、處理及保存方法1.  血清：使用不含熱原和內(nèi)毒素的試管，操作過(guò)程中避免任何細(xì)胞刺激，收集血液后，3000轉(zhuǎn)離心10分鐘將血清和紅細(xì)胞迅速小心地分離。2.  血漿：EDTA、檸檬酸鹽或肝素抗凝。3000轉(zhuǎn)離心30分鐘取上清。3.  細(xì)胞上清液：3000轉(zhuǎn)離心10分鐘去除顆粒和聚合物。4.  組織勻漿：將組織加入適量生理鹽水搗碎。3000轉(zhuǎn)離心10分鐘取上清。5.  保存：如果樣本收集后不及時(shí)檢測(cè)，請(qǐng)按一次用量分裝，凍存于-20℃，避免反復(fù)凍融，在室溫下解凍并確保樣品均勻地充分解凍。自備物品1. 酶標(biāo)儀（450nm）2. 高精度加樣器及槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒溫箱操作注意事項(xiàng)1.  試劑盒保存在2-8℃，使用前室溫平衡20分鐘。從冰箱取出的濃縮洗滌液會(huì)有結(jié)晶，這屬于正常現(xiàn)象，水浴加熱使結(jié)晶完全溶解后再使用。2.  實(shí)驗(yàn)中不用的板條應(yīng)立即放回自封袋中，密封（低溫干燥）保存。3.  濃度為0的S0號(hào)標(biāo)準(zhǔn)品即可視為陰性對(duì)照或者空白；按照說(shuō)明書(shū)操作時(shí)樣本已經(jīng)稀釋5倍，＊終結(jié)果乘以5才是樣本實(shí)際濃度。4.  嚴(yán)格按照說(shuō)明書(shū)中標(biāo)明的時(shí)間、加液量及順序進(jìn)行溫育操作。5.  所有液體組分使用前充分搖勻。試劑盒組成名稱(chēng)96孔配置48孔配置備注微孔酶標(biāo)板12孔×8條12孔×4條無(wú)標(biāo)準(zhǔn)品0.3mL*6管0.3mL*6管無(wú)樣本稀釋液6mL3mL無(wú)檢測(cè)抗體-HRP10mL5mL無(wú)20×洗滌緩沖液25mL15mL按說(shuō)明書(shū)進(jìn)行稀釋底物A6mL3mL無(wú)底物B6mL3mL無(wú)終止液6mL3mL無(wú)封板膜2張2張無(wú)說(shuō)明書(shū)1份1份無(wú)自封袋1個(gè)1個(gè)無(wú)注：標(biāo)準(zhǔn)品（S0-S5）濃度依次為：0、7.5、15、30、60、120 pg/mL試劑的準(zhǔn)備 20×洗滌緩沖液的稀釋?zhuān)赫麴s水按1：20稀釋?zhuān)?份的20×洗滌緩沖液加19份的蒸餾水。洗板方法1.  手工洗板：甩盡孔內(nèi)液體，每孔加滿(mǎn)洗滌液，靜置1min后甩盡孔內(nèi)液體，在吸水紙上拍干，如此洗板5次。2.  自動(dòng)洗板機(jī)：每孔注入洗液350μL，浸泡1min，洗板5次。操作步驟1.  從室溫平衡20min后的鋁箔袋中取出所需板條，剩余板條用自封袋密封放回4℃。2.  設(shè)置標(biāo)準(zhǔn)品孔和樣本孔，標(biāo)準(zhǔn)品孔各加不同濃度的標(biāo)準(zhǔn)品50μL；3.  樣本孔先加待測(cè)樣本10μL，再加樣本稀釋液40μL；空白孔不加。4.  除空白孔外，標(biāo)準(zhǔn)品孔和樣本孔中每孔加入辣根過(guò)氧化物酶（HRP）標(biāo)記的檢測(cè)抗體100μL，用封板膜封住反應(yīng)孔，37℃水浴鍋或恒溫箱溫育60min。5.  棄去液體，吸水紙上拍干，每孔加滿(mǎn)洗滌液，靜置1min，甩去洗滌液，吸水紙上拍干，如此重復(fù)洗板5次（也可用洗板機(jī)洗板）。6.  每孔加入底物A、B各50μL，37℃避光孵育15min。7.  每孔加入終止液50μL，15min內(nèi)，在450nm波長(zhǎng)處測(cè)定各孔的OD值。結(jié)果判斷 繪制標(biāo)準(zhǔn)曲線(xiàn)：在Excel工作表中，以標(biāo)準(zhǔn)品濃度作橫坐標(biāo)，對(duì)應(yīng)OD值作縱坐標(biāo)，繪制出標(biāo)準(zhǔn)品線(xiàn)性回歸曲線(xiàn)，按曲線(xiàn)方程計(jì)算各樣本濃度值。試劑盒性能1.  準(zhǔn)確性：標(biāo)準(zhǔn)品線(xiàn)性回歸與預(yù)期濃度相關(guān)系數(shù)R值，大于等于0.9900。2.  靈敏度：＊低檢測(cè)濃度小于1.0 pg/mL。3.  特異性：不與其它可溶性結(jié)構(gòu)類(lèi)似物交叉反應(yīng)。4.  重復(fù)性：板內(nèi)、板間變異系數(shù)均小于15%。5.  貯藏：2-8℃，避光防潮保存。6.  有效期：6個(gè)月免責(zé)聲明1.   試劑盒僅供研究使用，不得用于臨床實(shí)驗(yàn)或人體實(shí)驗(yàn)，否則所產(chǎn)生的一切后果，由實(shí)驗(yàn)者承擔(dān)，本公司概不負(fù)責(zé)。2.   嚴(yán)格按照說(shuō)明書(shū)操作，實(shí)驗(yàn)者違反說(shuō)明書(shū)操作，后果由實(shí)驗(yàn)者承擔(dān)。  FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Human Interleukin 2 (IL-2) ELISA Kit instruction Intended useThis IL-2 ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IL-2 in the sample, this IL-2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IL-2 concentration. The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sample collection and storagesSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cyclesPlasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Note:  The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Materials required but not supplied1.  Standard microplate reader(450nm)2.  Precision pipettes and Disposable pipette tips.3.  37 ℃ incubatorPrecautions1.  Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.2.  Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.3.  Mix all reagents before using.Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)Materials suppliedName96 determinations48 determinationsMicroelisa stripplate12*8strips12*4stripsStandard0.3ml*6tubes0.3ml*6tubesSample Diluent6.0ml3.0mlHRP-Conjugate reagent10.0ml5.0ml20X Wash solution25ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution B6.0ml3.0mlStop Solution6.0ml3.0mlClosure plate membrane22User manual11Sealed bags11Note: Standard (S0 → S5) concentration was followed by:0,7.5,15,30,60,120 pg/mlReagent preparation20×wash solution:Dilute with Distilled or deionized water 1:20.Assay procedure1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.2.  Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.3.  Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.4.  Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 5.  Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.6.  Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.7.  Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.8.  Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.Calculation of results1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.5. The sensitivity by this assay is 1.0 pg/ml6. Standard curve Storage：  2-8℃.validity： six months.  FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

本試劑盒只能用于科學(xué)研究，不得用于醫(yī)學(xué)診斷人（Human）γ干擾素（IFN-γ）ELISA檢測(cè)試劑盒使用說(shuō)明書(shū)檢測(cè)原理試劑盒采用雙抗體一步夾心法酶聯(lián)免疫吸附試驗(yàn)（ELISA）。往預(yù)先包被γ干擾素（IFN-γ）抗體的包被微孔中，依次加入標(biāo)本、標(biāo)準(zhǔn)品、HRP標(biāo)記的檢測(cè)抗體，經(jīng)過(guò)溫育并徹底洗滌。用底物TMB顯色，TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成＊終的黃色。顏色的深淺和樣品中的γ干擾素（IFN-γ）呈正相關(guān)。用酶標(biāo)儀在450nm 波長(zhǎng)下測(cè)定吸光度（OD 值），計(jì)算樣品濃度。樣品收集、處理及保存方法1.  血清：使用不含熱原和內(nèi)毒素的試管，操作過(guò)程中避免任何細(xì)胞刺激，收集血液后，3000轉(zhuǎn)離心10分鐘將血清和紅細(xì)胞迅速小心地分離。2.  血漿：EDTA、檸檬酸鹽或肝素抗凝。3000轉(zhuǎn)離心30分鐘取上清。3.  細(xì)胞上清液：3000轉(zhuǎn)離心10分鐘去除顆粒和聚合物。4.  組織勻漿：將組織加入適量生理鹽水搗碎。3000轉(zhuǎn)離心10分鐘取上清。5.  保存：如果樣本收集后不及時(shí)檢測(cè)，請(qǐng)按一次用量分裝，凍存于-20℃，避免反復(fù)凍融，在室溫下解凍并確保樣品均勻地充分解凍。自備物品1. 酶標(biāo)儀（450nm）2. 高精度加樣器及槍頭：0.5-10uL、2-20uL、20-200uL、200-1000uL3. 37℃恒溫箱操作注意事項(xiàng)1.  試劑盒保存在2-8℃，使用前室溫平衡20分鐘。從冰箱取出的濃縮洗滌液會(huì)有結(jié)晶，這屬于正常現(xiàn)象，水浴加熱使結(jié)晶完全溶解后再使用。2.  實(shí)驗(yàn)中不用的板條應(yīng)立即放回自封袋中，密封（低溫干燥）保存。3.  濃度為0的S0號(hào)標(biāo)準(zhǔn)品即可視為陰性對(duì)照或者空白；按照說(shuō)明書(shū)操作時(shí)樣本已經(jīng)稀釋5倍，＊終結(jié)果乘以5才是樣本實(shí)際濃度。4.  嚴(yán)格按照說(shuō)明書(shū)中標(biāo)明的時(shí)間、加液量及順序進(jìn)行溫育操作。5.  所有液體組分使用前充分搖勻。試劑盒組成名稱(chēng)96孔配置48孔配置備注微孔酶標(biāo)板12孔×8條12孔×4條無(wú)標(biāo)準(zhǔn)品0.3mL*6管0.3mL*6管無(wú)樣本稀釋液6mL3mL無(wú)檢測(cè)抗體-HRP10mL5mL無(wú)20×洗滌緩沖液25mL15mL按說(shuō)明書(shū)進(jìn)行稀釋底物A6mL3mL無(wú)底物B6mL3mL無(wú)終止液6mL3mL無(wú)封板膜2張2張無(wú)說(shuō)明書(shū)1份1份無(wú)自封袋1個(gè)1個(gè)無(wú)注：標(biāo)準(zhǔn)品（S0-S5）濃度依次為：0、50、100、200、400、800 pg/mL試劑的準(zhǔn)備 20×洗滌緩沖液的稀釋?zhuān)赫麴s水按1：20稀釋?zhuān)?份的20×洗滌緩沖液加19份的蒸餾水。洗板方法1.  手工洗板：甩盡孔內(nèi)液體，每孔加滿(mǎn)洗滌液，靜置1min后甩盡孔內(nèi)液體，在吸水紙上拍干，如此洗板5次。2.  自動(dòng)洗板機(jī)：每孔注入洗液350μL，浸泡1min，洗板5次。操作步驟1.  從室溫平衡20min后的鋁箔袋中取出所需板條，剩余板條用自封袋密封放回4℃。2.  設(shè)置標(biāo)準(zhǔn)品孔和樣本孔，標(biāo)準(zhǔn)品孔各加不同濃度的標(biāo)準(zhǔn)品50μL；3.  樣本孔先加待測(cè)樣本10μL，再加樣本稀釋液40μL；空白孔不加。4.  除空白孔外，標(biāo)準(zhǔn)品孔和樣本孔中每孔加入辣根過(guò)氧化物酶（HRP）標(biāo)記的檢測(cè)抗體100μL，用封板膜封住反應(yīng)孔，37℃水浴鍋或恒溫箱溫育60min。5.  棄去液體，吸水紙上拍干，每孔加滿(mǎn)洗滌液，靜置1min，甩去洗滌液，吸水紙上拍干，如此重復(fù)洗板5次（也可用洗板機(jī)洗板）。6.  每孔加入底物A、B各50μL，37℃避光孵育15min。7.  每孔加入終止液50μL，15min內(nèi)，在450nm波長(zhǎng)處測(cè)定各孔的OD值。結(jié)果判斷 繪制標(biāo)準(zhǔn)曲線(xiàn)：在Excel工作表中，以標(biāo)準(zhǔn)品濃度作橫坐標(biāo)，對(duì)應(yīng)OD值作縱坐標(biāo)，繪制出標(biāo)準(zhǔn)品線(xiàn)性回歸曲線(xiàn)，按曲線(xiàn)方程計(jì)算各樣本濃度值。 試劑盒性能1.  準(zhǔn)確性：標(biāo)準(zhǔn)品線(xiàn)性回歸與預(yù)期濃度相關(guān)系數(shù)R值，大于等于0.9900。2.  靈敏度：＊低檢測(cè)濃度小于1.0 pg/mL。3.  特異性：不與其它可溶性結(jié)構(gòu)類(lèi)似物交叉反應(yīng)。4.  重復(fù)性：板內(nèi)、板間變異系數(shù)均小于15%。5.  貯藏：2-8℃，避光防潮保存。6.  有效期：6個(gè)月免責(zé)聲明1.   試劑盒僅供研究使用，不得用于臨床實(shí)驗(yàn)或人體實(shí)驗(yàn)，否則所產(chǎn)生的一切后果，由實(shí)驗(yàn)者承擔(dān)，本公司概不負(fù)責(zé)。2.   嚴(yán)格按照說(shuō)明書(shū)操作，實(shí)驗(yàn)者違反說(shuō)明書(shū)操作，后果由實(shí)驗(yàn)者承擔(dān)。  FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Human Interferon γ (IFN-γ) ELISA Kit instruction Intended useThis IFN-γ ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IFN-γ in the sample, this IFN-γ ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IFN-γ concentration. The concentration of IFN-γ in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sample collection and storagesSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cyclesPlasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Note:  The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Materials required but not supplied1.  Standard microplate reader(450nm)2.  Precision pipettes and Disposable pipette tips.3.  37 ℃ incubatorPrecautions1.  Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.2.  Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.3.  Mix all reagents before using.Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)Materials suppliedName96 determinations48 determinationsMicroelisa stripplate12*8strips12*4stripsStandard0.3ml*6tubes0.3ml*6tubesSample Diluent6.0ml3.0mlHRP-Conjugate reagent10.0ml5.0ml20X Wash solution25ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution B6.0ml3.0mlStop Solution6.0ml3.0mlClosure plate membrane22User manual11Sealed bags11Note: Standard (S0 → S5) concentration was followed by:0,50,100,200,400,800 pg/mlReagent preparation20×wash solution:Dilute with Distilled or deionized water 1:20.Assay procedure1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.2.  Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.3.  Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.4.  Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C. 5.  Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.6.  Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.7.  Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not appear uniform, gently tap the plate to ensure thorough mixing.8.  Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.Calculation of results1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. 2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. 3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. 4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.5. The sensitivity by this assay is 1.0 pg/ml6. Standard curve Storage：  2-8℃.validity： six months.  FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Catalog NoIsotypeReactivityApplicationsGene Name Protein NameImmunogen Specificity FormulationSource PurificationDilution Concentration PurityStorage Stability Synonyms Observed Band Cell PathwayTissue SpecificityDM-Ab-13828IgG Human;Mouse;Rat IHC;IFCD68MacrosialinSynthetic Peptide of CD68The antibody detects endogenous CD68 proteins.PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.Monoclonal, MouseThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.WB 500-2000 1:200 IF 1:50-200 1 mg/ml≥90%-20°C/1 yearCD68; Macrosialin; Gp110; CD6837kD[IsoformShort]: Cell membrane; Single-pass type I membrane protein.; [Isoform Long]: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein.Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor celllines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.                  

EID2B antibody Product Information Catalog No. : DM02652 Size: 100μg Form: liquid Purification: Immunogen affinity purified Purity: ≥95% as determined by SDS-PAGEHost: Rabbit Clonality: polyclonal Clone ID: None IsoType: IgGStorage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) Background       Acts as a repressor of MYOD-dependent transcription, glucocorticoid receptor-dependent transcription, and muscle differentiation.Immunogen information      Immunogen: EP300 interacting inhibitor of differentiation 2B Synonyms: EP300-interacting inhibitor of differentiation 2B (EID-2B)|EID-2-like inhibitor of differentiation 3 (EID-3)|EID2B|EID3 Observed MW: 43 kDa Uniprot ID : Q96D98Application       Reactivity: Human Tested Application: ELISA, WB Recommended dilution:WB: 1:500-1:2000

ATF-3 Polyclonal Antibody Catalog No DM-Ab-01554 Isotype IgG Reactivity Human;Mouse;Rat Applications WB;IHC;IF;ELISA Gene Name ATF3Protein Name Cyclic AMP-dependent transcription factor ATF-3 Immunogen The antiserum was produced against synthesized peptide derived from human ATF3. AA range:131-180 Specificity ATF-3 Polyclonal Antibody detects endogenous levels of ATF-3 protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgGPurification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. Dilution WB 1:500-2000;IHC-p 1:100-500;IF/ICC 1:100-500;ELISA 1:5000-20000 Concentration 1 mg/ml Purity ≥90% Storage Stability -20°C/1 yearSynonyms ATF3; Cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; Activating transcription factor 3 Observed Band 21kD Cell Pathway Nucleus . Tissue Specificity Amygdala,Brain,Cerebellum,Muscle,Function function:This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. ATF subfamily.,similarity:Contains 1 bZIP domain.,subunit:Binds DNA as a homodimer or a heterodimer.,Background activating transcription factor 3(ATF3) Homo sapiens This gene encodes a possible that alternative splicing of this gene may be physiologically important in matters needing attention Avoid repeated freezing and thawing!Usage suggestions This product can be used in immunological reaction related experiments. For Western Blot analysis of 293T-UV cells using ATF-3 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). Western blot analysis of lysates from RAW264.7 cells, using ATF3 Antibody. The lane on the right is blocked with the synthesized peptide. member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is more information, please consult technical personnel. the regulation of target genes. [provided by RefSeq, Apr 2011],Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). 

產(chǎn)品描述

香菇多糖       香菇多糖（Lentinan，簡(jiǎn)稱(chēng)LNT）是一種從優(yōu)質(zhì)香菇子實(shí)體中提取的有效活性成分，也是香菇的主要有效成分，它屬于多糖類(lèi)物質(zhì)。中文名稱(chēng)：香菇多糖中文別名：香菇多糖;瘤停能;香菇糖;Lentinan 香菇多糖;香菇粉;香菇提取物;云芝多糖;豬苓多糖;香菇菌多糖英文名稱(chēng)：Lentinan英文別名：Lentinan;LC 33;biomoduline;LENTINAN (SHIITAKE MUSHROOM POLYSACCHARIDES);LENTINEX(R);lentinan vial;beta-D-glucopyranosyl-(1->6)-[beta-D-glucopyranosyl-(1->3)-[beta-D-glucopyranosyl-(1->6)]-beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->3)-beta-D-glucopyranosyl-(1->3)]-beta-D-glucopyranose;Bromoduline;DRG-0171;LC-33;Lentinan polysaccharide;Polysacharid lentinus edodes;β(1-3) linked D-glucose with 2 side chains of single β(1-6) D-glucose every five unit.;BiomodulineCAS號(hào)：37339-90-5分子式：C42H72O36分子量：1153.00香菇多糖英文名：LentinanCAS號(hào)：37339-90-5純度：BR,50%沸點(diǎn)： 1472°Cat760mmHg比旋光度： D20 +13.5-14.5° (in 2% NaOH); +19.5-21.5° (in 10% NaOH)溶解性： 溶于堿溶液或甲酸，微溶于熱水或二甲亞砜，不溶于冷水、醇、乙醚、氯仿、吡啶或六甲基磷酰胺。儲(chǔ)存條件： RT化學(xué)組成與性質(zhì)化學(xué)組成：香菇多糖主要以β-D(1→3)葡聚糖殘基為主鏈，側(cè)鏈為(1→6)葡聚糖殘基的葡聚糖。其活性成分是具有分支的β—(1—3)—D—葡聚糖，主鏈由β—(1—3)—連接的葡萄糖基組成，沿主鏈隨機(jī)分布著由β—(1—6)連接的葡萄糖基，呈梳狀結(jié)構(gòu)。性質(zhì)：香菇多糖為灰白色粉末，大多為酸性多糖，溶于水、稀堿，尤其易溶于熱水，不溶于乙醇、丙酮、乙酸乙酯、乙醚等有機(jī)溶劑。其水溶液呈透明黏稠狀。功效與作用抗病毒：香菇多糖能夠提高感染細(xì)胞的免疫力，增強(qiáng)細(xì)胞膜的穩(wěn)定性，抑制細(xì)胞病變，促進(jìn)細(xì)胞修復(fù)，從而起到抗病毒的作用。它可以用于乙型肝炎和艾滋病等病毒性疾病的治療?？鼓[瘤：香菇多糖是一種宿主免疫增強(qiáng)劑，雖然它本身在體內(nèi)無(wú)直接殺傷腫瘤細(xì)胞的作用，但可以通過(guò)增強(qiáng)機(jī)體的免疫功能而發(fā)揮抗腫瘤活性。它能增強(qiáng)脾臟和腹腔的NK細(xì)胞活性，誘生干擾素，與白細(xì)胞介素類(lèi)或干擾素誘導(dǎo)劑有協(xié)同作用。香菇多糖主要用于不能手術(shù)或復(fù)發(fā)性胃癌、肝癌、膀胱癌等腫瘤患者的輔助治療，能夠緩解癥狀，提高病人免疫功能，糾正微量元素失調(diào)。調(diào)節(jié)免疫功能：香菇多糖能夠識(shí)別脾臟及肝臟中抗原的巨噬細(xì)胞，促進(jìn)淋巴細(xì)胞活化因子的產(chǎn)生，釋放各種輔助性T細(xì)胞因子，增強(qiáng)宿主腹腔巨噬細(xì)胞吞噬率，從而調(diào)節(jié)機(jī)體的免疫功能。其他作用：香菇多糖還具有降血壓、降血脂等生理功能，并可用于治療具有抗藥性的肺結(jié)核和老年性慢性支氣管炎等疾病。提取與純化       香菇多糖的提取多采用熱水及稀堿溶液，避免在強(qiáng)酸、堿溶液中進(jìn)行，以防止多糖中糖苷鍵斷裂及構(gòu)象變化。常用的分離純化方法包括沸水浸提、乙醇沉淀、透析及柱層析等步驟。

產(chǎn)品描述

Stat3 Polyclonal AntibodyCatalog No DM-Ab-02054Isotype IgGReactivity Human;Mouse;RatApplications IF;WB;IHC;ELISAGene Name STAT3Protein Name Signal transducer and activator of transcription 3Immunogen The antiserum was produced against synthesized peptide derived from humanSTAT3. AA range:672-721Specificity Stat3 Polyclonal Antibody detects endogenous levels of Stat3 protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgGPurification The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific immunogen. Dilution IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Concentration 1 mg/mlPurity ≥90%Storage Stability -20°C/1 yearSynonyms STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phaseresponse factorObserved Band 88kDCell Pathway Cytoplasm . Nucleus . Shuttles between the nucleus and the cytoplasm. Tissue Specificity Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Function     disease:Defects in STAT3 are the cause of hyperimmunoglobulin E recurrentELISA: 1/5000. Not yet tested in other applications.Translocated into the nucleus upon tyrosine phosphorylation and dimerization, inresponse to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Expressed in naive CD4(+) T cells as well as T-helper Th17, Th1 and Th2 cells(PubMed:31899195). Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitoryfactor (LIF) stimulation, accumulates in the nucleus. The complex composed ofBART and ARL2 plays an important role in the nuclear translocation and retentioninfection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known ashyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunityand connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctivecoarse facial appearance, abnormal dentition, hyperextensibility of the joints, andbone fractures.,function:Transcription factor that binds to the interleukin-6of STAT3. Identified in a complex with LYN and PAG1.Jiao, Z., Ma, Y., Zhang, Q. et al. The adipose-derivedmesenchymal stem cell secretome promotes hepaticregeneration in miniature pigs after liverischaemia-reperfusion combined with partialresection. Stem Cell Res Ther 12, 218 (2021).Liu, Bowen, et al. "Oncoprotein HBXIP enhancesHOXB13 acetylation and co-activates HOXB13 toconfer tamoxifen resistance in breast cancer." Journalof hematology & oncology 11.1 (2018): 26.Immunofluorescence analysis of Hela cell. 1,Stat3Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-tubulin Monoclonal Antibody(M7)(green)Liu, Yanmei, et al. "Cancer Stem Cells are Regulatedby STAT3 Signalling in Wilms Tumour." Journal ofCancer 9.8 (2018): 1486.was diluted at 1:200(4° overnight). 2, Goat Anti RabbitAlexa Fluor 594 Catalog:RS3611 was diluted at1:1000(room temperature, 50min). Goat Anti MouseAlexa Fluor 488 Catalog:RS3208 was diluted at1:1000(room temperature, 50min).

IL-6 Polyclonal AntibodyCatalog No  DM-Ab-16022Isotype         IgGReactivity         Human;Rat;MouseApplications         WB;IHC;IF;ELISAGene Name IL6Protein Name Interleukin-6Immunogen The antiserum was produced against synthesized peptide derived from theInternal region of human IL6. AA range:131-180Specificity IL-6 Polyclonal Antibody detects endogenous levels of IL-6 protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgGPurification The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific immunogen. Dilution Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300Concentration 1 mg/mlPurity ≥90%Storage Stability -20°C/1 yearSynonyms IL6; IFNB2; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTLdifferentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2Observed Band 23kDCell Pathway Secreted . Tissue Specificity Produced by skeletal muscle. Function disease:At least 1 polymorphism in the IL6 gene renders HIV-infected menNot yet tested in other applications.susceptible to Kaposi sarcoma [MIM:148000]. Kaposi sarcoma is a sarcoma ofspindle cells mixed with angiomatous tissue. A relatively rare malignant skintumor that results in multifocal purplish coloured papules or plaques thateventually form nodules. Most commonly seen in patients who suffer fromAIDS.,disease:Genetic variations in IL6 are associated with susceptibility tosystemic juvenile rheumatoid arthritis [MIM:604302]. Systemic juvenilerheumatoid arthritis is juvenile chronic arthritis associated with severe, debilitating, extraarticular features, and occasionally fatal complications. Despitemedical treatment, many children still experience early joint destruction, necessitating surgical replacement.,function:IL-6 is a cytokine with a wide varietyof biological functions: it plays an essential role in theJiao, Z., Ma, Y., Zhang, Q. et al. The adipose-derivedmesenchymal stem cell secretome promotes hepaticregeneration in miniature pigs after liverischaemia-reperfusion combined with partialresection. Stem Cell Res Ther 12, 218 (2021)Liu, Bowen, et al. "Oncoprotein HBXIP enhancesHOXB13 acetylation and co-activates HOXB13 toconfer tamoxifen resistance in breast cancer." Journalof hematology & oncology 11.1 (2018): 26.Liu, Yanmei, et al. "Cancer Stem Cells are Regulatedby STAT3 Signalling in Wilms Tumour." Journal ofCancer 9.8 (2018): 1486.Immunofluorescence analysis of Hela cell. 1,Stat3Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-tubulin Monoclonal Antibody(M7)(green)Liu, Yanmei, et al. "Cancer Stem Cells are Regulatedby STAT3 Signalling in Wilms Tumour." Journal ofCancer 9.8 (2018): 1486.was diluted at 1:200(4° overnight). 2, Goat Anti RabbitAlexa Fluor 594 Catalog:RS3611 was diluted at1:1000(room temperature, 50min). Goat Anti MouseAlexa Fluor 488 Catalog:RS3208 was diluted at1:1000(room temperature, 50min).

OmnimAbs是什么品牌創(chuàng)新生物技術(shù)品牌OmnimAbs是一個(gè)創(chuàng)新生物技術(shù)品牌，專(zhuān)注于提供高質(zhì)量的單克隆抗體和抗原，服務(wù)于生命科學(xué)行業(yè)。OmnimAbs品牌自成立以來(lái)，已經(jīng)積累了10年的經(jīng)驗(yàn)，專(zhuān)注于供應(yīng)抗體和免疫診斷產(chǎn)品。其科學(xué)團(tuán)隊(duì)由單克隆抗體領(lǐng)域的專(zhuān)家組成，擁有在重組蛋白和熒光技術(shù)方面的專(zhuān)長(zhǎng)。這些專(zhuān)長(zhǎng)已經(jīng)轉(zhuǎn)化為產(chǎn)品和創(chuàng)新，為心力衰竭、腎衰竭、外科感染和腫瘤標(biāo)志物、HIV/趨化因子受體、信號(hào)轉(zhuǎn)導(dǎo)和神經(jīng)生物學(xué)等領(lǐng)域提供高質(zhì)量的單克隆抗體和抗原。OmnimAbs不僅提供產(chǎn)品，還為工業(yè)和研究應(yīng)用提供全面的支持?jǐn)?shù)據(jù)服務(wù)和便捷的產(chǎn)品搜索工具。此外，OmnimAbs還提供多種國(guó)際知名品牌的試劑與耗材，包括但不限于A(yíng)blab、Eurogentec、Chemicell、BrainBits、Emsdiasum、Dyesol、Bangs Laboratories、Macrocyclics、Flystuff、EYlabs、scientific Commodities、Xcessbio Biosciences、omicronbio、A-M systems、Glycotech、Diagnostic Automation、AK Scientific、emfret ANALYTICS、LaysanBio、Peptides International、ademtech、Skyspring nanomaterials、Biosearchtech、Hematologic Technologies等。這些品牌涵蓋了廣泛的領(lǐng)域，包括但不限于生物化學(xué)、分子生物學(xué)和細(xì)胞培養(yǎng)等。OmnimAbs還特別提到其進(jìn)口胎牛血清產(chǎn)品，這是一種來(lái)源于美國(guó)的熱滅活胎牛血清，適用于廣泛的細(xì)胞培養(yǎng)應(yīng)用，并經(jīng)過(guò)無(wú)菌過(guò)濾處理，適用于昆蟲(chóng)細(xì)胞培養(yǎng)，促進(jìn)穩(wěn)定、健康的細(xì)胞生長(zhǎng)。綜上所述，OmnimAbs不僅是一個(gè)提供高質(zhì)量生物技術(shù)產(chǎn)品的品牌，還致力于滿(mǎn)足生命科學(xué)領(lǐng)域的多樣化需求，通過(guò)其專(zhuān)業(yè)的團(tuán)隊(duì)和全面的產(chǎn)品支持，為科研人員和實(shí)驗(yàn)室提供便利和效率

PeproTech于1988年創(chuàng)立于美國(guó)新澤西州，經(jīng)過(guò)26年的發(fā)展，已成為世界上＊大的重組細(xì)胞因子和蛋白、相關(guān)抗體及ELISA試劑盒生產(chǎn)商之一。PeproTech公司已開(kāi)發(fā)出2,000多種產(chǎn)品，其精良的技術(shù)保證了產(chǎn)品的高質(zhì)量、高可靠性和高穩(wěn)定性。 Peprotech的產(chǎn)品具有很高的性?xún)r(jià)比，在世界上眾多高水平的實(shí)驗(yàn)室和研究機(jī)構(gòu)中廣泛使用。截至2013年底，已被13,000篇SCI文獻(xiàn)引用。

賽默飛世爾科技公司(Thermo Fisher Scientific)是一家總部位于馬薩諸塞州沃爾瑟姆的美國(guó)科技服務(wù)供應(yīng)商，在1956年由數(shù)個(gè)公司合并而成，其主要客戶(hù)類(lèi)型包括醫(yī)藥和生物公司，醫(yī)院和臨床診斷實(shí)驗(yàn)室，大學(xué)、科研院所和政府機(jī)構(gòu)，以及環(huán)境與工業(yè)過(guò)程控制裝備制造商等，為其提供用于研究、分析、發(fā)現(xiàn)和診斷的分析儀器、設(shè)備、試劑和耗材、軟件和服務(wù)。Thermo Scientific能夠提供一整套包括高端分析儀器、實(shí)驗(yàn)室裝備、軟件、服務(wù)、耗材和試劑在內(nèi)的實(shí)驗(yàn)室綜合解決方案。

圣克魯斯生物技術(shù)在生物醫(yī)藥研究市場(chǎng)的發(fā)展中處于＊＊＊＊地位。在過(guò)去的30年中,本公司已把重點(diǎn)放在不斷發(fā)展的研究單克隆抗體、生物化學(xué)、實(shí)驗(yàn)室器具和CRISPR產(chǎn)品。

Tocris Bioscience是一家專(zhuān)賣(mài)生命科學(xué)研究chemicals, peptides and antibodies的知名品牌，其產(chǎn)品也廣為各大藥廠(chǎng)、大學(xué)、研究機(jī)構(gòu)，超過(guò)五萬(wàn)名科學(xué)研究者所采用。Tocris bioscience是位于英國(guó)布里斯托爾（Bristol）的高品質(zhì)試劑提供商，共有2000多種產(chǎn)品，主要集中在神經(jīng)科學(xué)和信號(hào)傳導(dǎo)領(lǐng)域，產(chǎn)品類(lèi)型包括小分子、多肽、抗體、配體和化合物篩選文庫(kù)等，主要產(chǎn)品包括GPCR ligands，神經(jīng)傳遞素，離子通道調(diào)控劑，信號(hào)通路抑制劑等，這些產(chǎn)品被廣泛選擇性地用于阻斷或激活生物學(xué)通路。

R&D細(xì)胞因子，R&D ELISA試劑盒，RD試劑產(chǎn)品描述：我公司專(zhuān)業(yè)代理R&D產(chǎn)品，產(chǎn)品多樣，涉及細(xì)胞因子，生物試劑，ELISA試劑盒。保證原裝，價(jià)格，貨期快，服務(wù)好！

QIAGEN，是全球＊＊的樣品制備和檢測(cè)技術(shù)供應(yīng)商。樣品制備技術(shù)用來(lái)分離和處理從血液或組織等生物樣品中的DNA，RNA和蛋白質(zhì)，檢測(cè)技術(shù)使這些分離的分子，在隨后的分析中顯現(xiàn)，如特定病毒的DNA。其使命是使客戶(hù)在生命科學(xué)，應(yīng)用測(cè)試，制藥，分子診斷等方面實(shí)現(xiàn)卓越的成就和突破。

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Invitrogen是全球＊＊的生命技術(shù)公司，公司創(chuàng)立于1987年，總部位于美國(guó)加利福尼亞州卡爾斯巴德。主要為全球從事生命科學(xué)研究的學(xué)術(shù)、政府研究機(jī)構(gòu)、醫(yī)藥和生物技術(shù)公司提供生物技術(shù)產(chǎn)品和服務(wù)。Invitrogen將研發(fā)力量集中在包括功能基因組、蛋白組學(xué)、生物信息學(xué)和細(xì)胞生物學(xué)等生命科學(xué)研究領(lǐng)域內(nèi)的突破性創(chuàng)新，向全球各主要實(shí)驗(yàn)室提供在研究中所需要的新技術(shù)、新產(chǎn)品及服務(wù)。 Invitrogen旗下包括Invitrogen、Gibco、MolecularProbes、Dynal、Biosource、Caltag、Zymed、Panvera、InforMax、BioReliance等眾多行業(yè)＊＊＊＊。為分子生物、疾病研究，藥物研發(fā)和生物制品生產(chǎn)等提供全面的技術(shù)和服務(wù)。

Cell Signaling Technology (CST) 是美國(guó)＊＊的生物公司和細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)研究的＊＊，提供特色的信號(hào)轉(zhuǎn)導(dǎo)檢測(cè)用抗體及磷酸化、乙酰化、甲基化、泛素化抗體、ELISA試劑盒、細(xì)胞因子、化學(xué)試劑、激酶等產(chǎn)品。CST一家總部位于美國(guó)馬薩諸塞州丹弗斯的私營(yíng)公司,擁有專(zhuān)業(yè)的生產(chǎn)和研發(fā)隊(duì)伍，致力于提供全球高品質(zhì)創(chuàng)新型研究產(chǎn)品，以加速生物學(xué)認(rèn)知

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AnaSpec于1993年在美國(guó)成立，是一家由多肽合成、熒光標(biāo)記、抗體、樹(shù)脂合成專(zhuān)家團(tuán)隊(duì)組成的生物企業(yè)，為全世界的科研工作者提供全套的蛋白質(zhì)組學(xué)研究解決方案。

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AbcamAbcam位于英國(guó)的劍橋科學(xué)園，成立于1998年，為生命科學(xué)研究提供嚴(yán)格驗(yàn)證的抗體,試劑,蛋白,試劑盒。超過(guò)11萬(wàn)種優(yōu)質(zhì)高效的蛋白研究工具,提供數(shù)據(jù)表、多項(xiàng)驗(yàn)證并附有客戶(hù)評(píng)價(jià),現(xiàn)貨速達(dá),幫您更快完成實(shí)驗(yàn)。

BioVision. Inc. 位于美國(guó)加州，是世界上＊＊＊＊專(zhuān)著于凋亡和細(xì)胞信號(hào)研究的公司。其產(chǎn)品質(zhì)優(yōu)、價(jià)廉、包裝使用方便。BioVision致力于為研究者們提供＊好、＊新的研究工具而不斷擴(kuò)大產(chǎn)品及服務(wù)質(zhì)量。該公司提供用于檢測(cè)早、中、晚期凋亡的試劑，可以檢測(cè)凋亡發(fā)生的不同區(qū)域，包括：線(xiàn)粒體、胞漿、胞膜及胞核。

Cayman ChemicalCayman Chemical在位于密西根安娜堡（Ann Arbor, Michigan）66,000平方英尺的工廠(chǎng)生產(chǎn)并制作近6000多種不同的化合物、抗體及分析試劑盒。向全球科學(xué)工作者提供多研究領(lǐng)域的生化、免疫試劑和分析試劑盒，其產(chǎn)品被廣泛應(yīng)用于腫瘤、氧化氮、神經(jīng)學(xué)、凋亡、氧化性損傷、內(nèi)分泌學(xué)等不同研究領(lǐng)域。Cayman Chemical可提供用于檢測(cè)的特色試劑盒，也提供多種高質(zhì)量試劑